VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Effect of various conditions on the stability of DNA-methylases from Mycobacterium smegmatis butyricum cells

   
Sharkova E.V., Nikol'skaia I.I., Shomodi P., Fel'desh I., Debov S.S.
PubMed Id: 3705506
Year: 1986  Volume: 32  Issue: 2  Pages: 125-129
DNA-methylases from M. sm. butyricum, produced by means of isoelectrofocusing, were characterized by slow spontaneous variations in the activity with amplitude of 75-80% under conditions of storage in glycerol at -10 degrees. Effect of various conditions on stabilization of activity of adenine methylases MMbu4,2 and MMbu7,2 was studied. Alterations in the enzymatic activity were not found in presence of substances applied usually to stabilize the enzymes, blood serum albumin and cations of two valent metals as the values of the alterations occurred in the ranges of the enzyme activity variations.
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Sharkova, E. V., Nikol'skaia, I. I., Shomodi, P., Fel'desh, I., Debov, S. S. (1986). Effect of various conditions on the stability of DNA-methylases from Mycobacterium smegmatis butyricum cells. Voprosy Meditsinskoi Khimii, 32(2), 125-129.
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