VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism

   
Makarov S.A., Kudriavtseva G.V., Shishkin V.I.
PubMed Id: 3811277
Year: 1986  Volume: 32  Issue: 6  Pages: 126-128
A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.
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Makarov, S. A., Kudriavtseva, G. V., Shishkin, V. I. (1986). Polarographic method for determining activity of dehydrogenases of the pentosephosphate pathway of carbohydrate metabolism. Voprosy Meditsinskoi Khimii, 32(6), 126-128.
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