VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

A modified highly sensitive spectrophotometric method for determining acid phosphatase activity in the animal liver

   
Siatkin S.P., Frolov V.A.
PubMed Id: 3811279
Year: 1986  Volume: 32  Issue: 6  Pages: 132-134
A modified spectrophotometric procedure is described for estimation of acid phosphatase activity in rabbit liver tissue. Coefficient of instrumental sensitivity (SA/C = 0.029), apparent molar absorption (epsilon 820 = 30,500), relative sensitivity of measurement (Cmin = 0.18 mM) were calculated as well as main metrological patterns were evaluated, which showed that the procedure was highly sensitive, accurate and reproducible. Standard error did not exceed 1% and standard deviation was not above 5%.
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Siatkin, S. P., Frolov, V. A. (1986). A modified highly sensitive spectrophotometric method for determining acid phosphatase activity in the animal liver. Voprosy meditsinskoi khimii, 32(6), 132-134.
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