Phase alterations in the intensity of gluconeogenesis were observed in 92 rats subjected to hypokinesia within 1-30 days. At early periods of fixation of the animals gluconeogenesis was increased in all the tissues studied; the activation was especially distinct within the first 7 days of the experiment. Intensity of gluconeogenesis was similar to control values within 15 days of hypokinesia and a slight activation was detected within 30 days. Content of glucose was maximal in liver tissue within 3 days but the glycogen level was distinctly decreased in all the periods of experiments except of the 7th day. The pattern of gluconeogenesis in liver tissue as well as content of carbohydrates should be taken into consideration in the course of prophylaxis and treatment of the unfavourable after-effects of hypokinesia.