VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Colorimetric method of sequential determination of several dehydrogenases in a smear of human leukocytes

   
Beliakovich A.G., Lakhina L.V., Pankratova E.V., Teplitskiĭ B.I., Chumakova G.P.
PubMed Id: 3630006
Year: 1987  Volume: 33  Issue: 3  Pages: 118-120
Activity of several dehydrogenases was estimated by means of sequential incubation of a smear of human leukocytes in various media containing specific substrates for each enzyme and p-nitrotetrazolium violet as an acceptor of electrons. Photometry of the coloured products of redox reactions was carried out in the photoelectrocolorimeter, which was used for registration of steady state kinetics of all the reactions studied under usual conditions. Relative activity of succinate-alpha-glycerophosphate dehydrogenases and NADH/alpha-glycerophosphate dehydrogenases was estimated with an error of 2-4% in individual donors, while deviations of these values in leukocytes of the donors constituted +/- 15% as compared with their middle level calculated in 32 donors.
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Beliakovich, A. G., Lakhina, L. V., Pankratova, E. V., Teplitskiĭ, B. I., Chumakova, G. P. (1987). Colorimetric method of sequential determination of several dehydrogenases in a smear of human leukocytes. Voprosy Meditsinskoi Khimii, 33(3), 118-120.
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