VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Physico-chemical characteristics of sorbitol dehydrogenase from the cytoplasm of bovine liver cells

   
Sudovtsov V.E., Zharmukhamedova T.Yu.
PubMed Id: 3630023
Year: 1987  Volume: 33  Issue: 3  Pages: 81-84
Partially purified preparation of sorbitol dehydrogenase, isolated from hepatocytes of bovine liver tissue, was active at a wide range of pH exhibiting the maximal activity at pH 9.0 in presence of NAD but not of NADP. The high rate of sorbitol and xylitol dehydration was observed, whereas the enzyme dehydrated ribitol at the 4-fold lower rate. Disc electrophoresis of the preparation in polyacrylamide gel, where sorbitol, xylitol and ribitol were used as substrates for colorimetric detection of the enzyme activity, exhibited six enzymatic zones with Rf 0.387, 0.266, 0.338, 0.193, 0.129 and 0.064. Optimal conditions were developed for storage of the active enzyme.
Download PDF:  
Citation:

Sudovtsov, V. E., Zharmukhamedova, T. Yu. (1987). Physico-chemical characteristics of sorbitol dehydrogenase from the cytoplasm of bovine liver cells. Voprosy Meditsinskoi Khimii, 33(3), 81-84.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)
◄        ►