VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

A method of isolating plasma membranes from adipose tissue of rats without preliminary treatment with collagenase

   
Egutkin G.G.
PubMed Id: 2833029
Year: 1987  Volume: 33  Issue: 6  Pages: 132-135
A rapid procedure has been described for preparation of a relatively pure fraction of plasmatic membranes from adipose tissue without treatment with collagenase. Gentle homogenization of rat fatty tissue in a buffered sucrose solution yielded membrane fractions that could be separated from the bulk of contaminating mitochondria and microsomes by a series of differential and isopycnic centrifugations. The preparation obtained was enriched 12-fold with 5'-nucleotidase as compared with the initial homogenate and contained minimal contaminations with mitochondria or elements of endoplasmic reticulum.
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Egutkin, G. G. (1987). A method of isolating plasma membranes from adipose tissue of rats without preliminary treatment with collagenase. Voprosy Meditsinskoi Khimii, 33(6), 132-135.
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