VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test

   
Goncharov N.P., Katsiya G.V., Butnev V.Yu., Vorontsov V.I., Khagundiukova L.B., Veseman R.
PubMed Id: 3369121
Year: 1988  Volume: 34  Issue: 1  Pages: 130-123
A radioimmunoassay of II-deoxycortisol in human and animal blood plasma was developed. The antiserum used in the method was prepared against a conjugate of II-deoxycortisol-3(0-carboxymethyloxime) and bovine serum albumin. Specificity of the antiserum was determined in cross reactions using 32 steroids. High specificity of the antiserum allowed to determine II-deoxycortisol without preliminary chromatographic isolation. Sensitivity of the method enabled to detect 25 pg of II-deoxycortisol. The intra- and interassay variations were less than 10% and 15%, respectively. The method was used for evaluation of hypophysis-adrenal cortex system functions under the conditions of metopirone test.
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Goncharov, N. P., Katsiya, G. V., Butnev, V. Yu., Vorontsov, V. I., Khagundiukova, L. B., Veseman, R. (1988). The use of a radioimmunologic method of analysis of 11-deoxycortisol for the evaluation of the function of the pituitary-adrenal cortex system during the metopirone test. Voprosy Meditsinskoi Khimii, 34(1), 130-123.
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