VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Radioimmunological analysis of 17 beta-estradiol and the effect of physical exertion on its levels in human blood

   
Morozov V.I., Priiatkin S.A., Isakova T.I., Lushchitskaia I.M., Rogozkin V.A.
PubMed Id: 3420801
Year: 1988  Volume: 34  Issue: 3  Pages: 136-139
Radioimmunoassay (RIA) for estradiol-17 beta (E2) in blood serum was developed. Antiserum against E2 was raised by means of rabbit immunization with 6-(O-carboxymethyl)oxime E2-BSA. Cross reactions of antiserum were studied with 22 natural and synthetic steroids. Antiserum was used at final dilution 1: 75,000. The sensitivity of RIA was 3 pg/tube, the detection efficiency was 83-120%, when 25-800 pg/ml E2 was added. The variation coefficients were 7.8% and 13% (intraassay and interassay, respectively). As shown by the RIA the intensive physical exercises resulted in the increase of E2 concentration by 26% in male athletes blood serum.
Download PDF:  
Citation:

Morozov, V. I., Priiatkin, S. A., Isakova, T. I., Lushchitskaia, I. M., Rogozkin, V. A. (1988). Radioimmunological analysis of 17 beta-estradiol and the effect of physical exertion on its levels in human blood. Voprosy Meditsinskoi Khimii, 34(3), 136-139.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)
◄        ►