VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Partial purification of tissue thromboplastin by the gel filtration method and its use for reproducing the generalized decompensated thrombinogenesis syndrome

   
Magerovskiĭ Iu.V., Monastyrskiĭ V.A., Gaĭda A.V., Birka I.I., Voroniak M.I.
PubMed Id: 3420807
Year: 1988  Volume: 34  Issue: 3  Pages: 34-39
Tissue thromboplastin was purified using various chromatographic resins: Sephadex G-200, Ultragel AcA 34, Acrylex P-300 and Toyopearl HW-55F. The purity of the preparations obtained was evaluated by means of the ratio of the thromboplastin solutions absorbency at 540 nm and a protein concentration in the solution expressed as mg/ml. The most purified preparations of tissue thromboplastin were obtained after gel filtration on Toyopearl HW-55F. The thromboplastin preparation produced was used for experimental reproduction of the syndrome of generalized decompensated thrombinogenesis.
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Magerovskiĭ, Iu. V., Monastyrskiĭ, V. A., Gaĭda, A. V., Birka, I. I., Voroniak, M. I. (1988). Partial purification of tissue thromboplastin by the gel filtration method and its use for reproducing the generalized decompensated thrombinogenesis syndrome. Voprosy Meditsinskoi Khimii, 34(3), 34-39.
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