VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin

   
Toshchakov V.Yu., Danilov M.A., Shestakova N.K., Kozlovskaia T.I., Kulikova E.Yu.
PubMed Id: 2479173
Year: 1989  Volume: 35  Issue: 4  Pages: 134-137
Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.
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Toshchakov, V. Yu., Danilov, M. A., Shestakova, N. K., Kozlovskaia, T. I., Kulikova, E. Yu. (1989). Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin. Voprosy Meditsinskoi Khimii, 35(4), 134-137.
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