VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Analogy and differences in properties of soluble forms of guanylate cyclase of the heart and rat blood platelets

   
Miroshnichenko V.P., Busygina O.G., Severina I.S.
PubMed Id: 2573196
Year: 1989  Volume: 35  Issue: 4  Pages: 60-66
Activity of guanylate cyclase in rat thrombocytes exceeded the enzymatic activity in heart tissue 3.2- and 6.6-fold, if Mn2+ and Mg2+ were used as cofactors, respectively. Dithiothreitol (DTT) at concentrations 2 x 10(-5) M-2 x 10(-2) M activated guanylate cyclase both in rat heart and thrombocytes, while 2 x 10(-3) M of DTT exhibited the maximal stimulating effect: 3-fold in heart tissue and 4.5-fold in thrombocytes. Only slight 2-fold activation of guanylate cyclase was observed in myocardium in presence of 1 x 10(-4) M nitroprusside, whereas this effect was distinctly augmented up to 26-fold after preincubation of the enzyme with 1 x 10(-4) M of nitroprusside within 45 min at 4 degrees in presence of 2 x 10(-4) M DTT. The stimulating effect of nitroprusside was increased up to 52-fold after addition of 3 micrograms hemoglobin into the sample. Nitroprusside did not show any stimulating effect on the guanylate cyclase activity in rat thrombocytes under experimental conditions used. Possible causes of the phenomenon observed are discussed.
Download PDF:  
Citation:

Miroshnichenko, V. P., Busygina, O. G., Severina, I. S. (1989). Analogy and differences in properties of soluble forms of guanylate cyclase of the heart and rat blood platelets. Voprosy Meditsinskoi Khimii, 35(4), 60-66.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)
◄        ►