VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Immunoaffinity purification of alpha 1-protease inhibitor from human plasma

   
Podiarene S.M., Letskene M.N., Mauritsas M.M., Planchiunene R.R.
PubMed Id: 2694602
Year: 1989  Volume: 35  Issue: 5  Pages: 96-99
A single-step procedure is described for isolation of alpha 1-inhibitor of proteases (alpha 1-IP) from human blood plasma using affinity chromatography on immunosorbent containing monoclonal antibodies against alpha 1-IP. Activity of the alpha 1-IP preparation, estimated by means of specific trypsin inhibition, was increased 61.1-fold after the chromatography, with a yield of the end product 20%. The preparation of alpha 1-IP obtained exhibited electrophoretic and immunochemical homogeneity.
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Podiarene, S. M., Letskene, M. N., Mauritsas, M. M., Planchiunene, R. R. (1989). Immunoaffinity purification of alpha 1-protease inhibitor from human plasma. Voprosy Meditsinskoi Khimii, 35(5), 96-99.
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