VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Isolation and purification of collagen proteins using biospecific chromatography on a sorbent with collagen-binding fragment of fibronectin

   
Bychkova V.V., Berman A.E.
PubMed Id: 2629236
Year: 1989  Volume: 35  Issue: 6  Pages: 129-133
A new procedure is described for isolation of collagens from various preparations containing small amounts of these proteins. The procedure is based on specific affinity of collagens of various types to one of peptide fragments of fibronectin--collagen-binding peptide (CBP). Collagens were isolated after single-step chromatography of tissue homogenate on the sorbent containing immobilized CBP. High specificity and reproducibility of the procedure was shown when various preparations were used: chicken embryo proteins with total radioactive label. Proteins synthesized in fibroblast culture; polypeptides synthesized in cell-free system of protein synthesis.
Download PDF:  
Citation:

Bychkova, V. V., Berman, A. E. (1989). Isolation and purification of collagen proteins using biospecific chromatography on a sorbent with collagen-binding fragment of fibronectin. Voprosy Meditsinskoi Khimii, 35(6), 129-133.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)