The role of divalent cations in DNA endonucleolysis catalyzed by restrictases

Sokolov N.N., Fitsner A.B., Anikeĭcheva N.V., Kovalenko N.A.
PubMed Id: 2629242
Year: 1989  Volume: 35  Issue: 6  Pages: 66-69
Electrophoretic analysis of products obtained after hydrolysis of phage lambda DNA by means of restrictase Pae I was carried out after preincubation of DNA or the enzyme with Mg2+ as well as after preincubation of DNA simultaneously with the enzyme and the subsequent addition of the required components into the experimental samples. The analysis showed that Mg2+ were apparently not required for the enzyme-substrate complex formation and caused destabilization of the complex. Restrictase Pae I was in active form when Mg2+ was substituted by Mn2+, Ca2+, Zn2+, Co2+ but not by Cd2+, Cu2+ or Ni2+. Experiments with o-phenanthroline showed that Zn2+ cations are of importance in catalytic activity of restrictase Pae I. Possible functions of Zn2+ in protein-nucleic acids recognition are discussed.
Download PDF:  

Sokolov, N. N., Fitsner, A. B., Anikeĭcheva, N. V., Kovalenko, N. A. (1989). The role of divalent cations in DNA endonucleolysis catalyzed by restrictases. Voprosy Meditsinskoi Khimii, 35(6), 66-69.
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)