VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Fluorescent method of determination of serum albumin in hyperbilirubinemia

   
Miller Yu.I., Yushko E.G., Dobretsov G.E., Krasovitsky B.M., Aĭdyraliev R.K.
PubMed Id: 2363267
Year: 1990  Volume: 36  Issue: 2  Pages: 86-88
A procedure is developed for estimation of human blood serum albumin in hyperbilirubinemia using a fluorescent probe 9,10-dianiline-2-sulfoanthracene (K-33). Use of the probe N-phenyl-I-amino-8-sulfonaphthalene (ANS) led to underestimation of albumin in hyperbilirubinemia as bilirubin and other inhibitors prevented the ANS binding with blood serum albumin. Substitution of ANS for K-33 enabled to estimate the albumin concentration under conditions of the pathology accompanied by an increase of endogenous ligands content in blood serum. The rate of K-33 (15 mM) fluorescence in blood serum (diluted 400-fold, at pH 4.0) correlated with albumin concentration (r-0.955).
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Miller Yu.I., Yushko E.G., Dobretsov G.E., Krasovitsky B.M., Aĭdyraliev R.K. (1990) Voprosy meditsinskoi khimii, 36(2), 86-88.
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