VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Characteristics of specific binding of thyroid hormones with plasma membranes of syncytiotrophoblast of human placenta

   
Karpyza E.I., Ermolenko M.N., Sviridov O.V.
PubMed Id: 2382428
Year: 1990  Volume: 36  Issue: 3  Pages: 45-48
Kinetic and equilibrium characteristics of the interaction of triiodothyronine (T3) and its structural analogues with plasmatic membranes of human placental syncytiotrophoblast were studied. The specific binding was temperature-dependent, saturable, reversible, selective and stereospecific. The following values of the binding parameters were obtained: association rate constant--1.0 x 10(10) M-1min-1, dissociation rate constant (fast phase)--2.4 x 10(-1) min-1, half-life time of the complex--2.9 min, equilibrium association constants for high- and low-affinity sites--2.0 x 10(10) M-1 and 4.8 x 10(6) M-1, respectively, concentrations of the corresponding sites--12.5 fmol and 36.0 pmol per mg of membrane protein, respectively. The affinity for membranes decreased in the following order: L = T3 greater than L = T4 greater than 3,3,5-triiodothyroacetic acid greater than 3,3,5-triiodothyropropionic acid greater than D = T3 greater than D = T4 greater than 3,5-diiodo-L-tyrosine.
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Karpyza, E. I., Ermolenko, M. N., Sviridov, O. V. (1990). Characteristics of specific binding of thyroid hormones with plasma membranes of syncytiotrophoblast of human placenta. Voprosy Meditsinskoi Khimii, 36(3), 45-48.
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