VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

The role of plasma high density lipoproteins as modulators of specific hormonal effect of hydrocortisone

   
Panin L.E., Svechnikova I.G., Maianskaia N.N.
PubMed Id: 2382432
Year: 1990  Volume: 36  Issue: 3  Pages: 60-62
Hydrocortisone affected less distinctly the gene expression in rat liver slices as compared with the simultaneous effect of the hormone and blood plasma high density lipoproteins (HDL). These substances acted cooperatively and the phenomenon observed was not reproduced by means of individual components. The protein moiety of HDL, but not of lipid part of the molecule, was of importance in realization of the effect. Inhibitors of lysosomal proteinases (pepstatin, soy bean inhibitor of trypsin and iodoacetamide) as well as inhibitors of lysosomes intracellular translocation (colchicine and vinblastine) blocked completely the cooperative action of hydrocortisone and HDL. Hydrocortisone-dependent activation of chromatin and stimulation of genes expression led to an increase in the binding constant of acridine orange with DNA, while molar concentration of binding sites of the probe was practically unaltered. At the same time, simultaneous effect of hydrocortisone and HDL did not alter the constants for acridine orange binding but concentration of the probe binding sites with DNA was distinctly increased.
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Panin, L. E., Svechnikova, I. G., Maianskaia, N. N. (1990). The role of plasma high density lipoproteins as modulators of specific hormonal effect of hydrocortisone. Voprosy Meditsinskoi Khimii, 36(3), 60-62.
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