Nucleoprotein-celite chromatography of cultivated cells nuclei, where incorporation of 3H-thymidine, 3H-UTP and 14C-aminohydrolysate into the cells was studied, enabled to detect that lowering of DNA-protein interactions in intact cells occurred simultaneously with a decrease in the rate of spontaneous macromolecular synthesis. The natural growth-regulating drugs, which inhibited distinctly DNA synthesis, inhibited RNA synthesis only slightly and stimulated protein synthesis within the first hours of the cells cultivation, prevented a decrease in tightness of DNA-protein bonds at the beginning of cultivation and then contributed to the reaction thus correlating with inhibition of RNA and protein synthesis. Definite interrelationship was found between alterations in the chromatin structural state and the cells functional activity. The cells lost their functional activity after pronounced decrease in tightness of DNA-protein bonds.
Svirnovskiĭ A.I. и др., Strength of nucleic acid-protein interactions and synthesis of nucleic acids and proteins in the cell //Voprosy meditsinskoi khimii.- 1991. -Т.37. -№ 1. С. 16-19.
Svirnovskiĭ A.I. и др., "Strength of nucleic acid-protein interactions and synthesis of nucleic acids and proteins in the cell." Voprosy meditsinskoi khimii 37.1 (1991): 16-19.
Svirnovskiĭ A.I., Tsygankov V.G., Ageĭchik V.M., Shimanskaia T.V. (1991). Strength of nucleic acid-protein interactions and synthesis of nucleic acids and proteins in the cell. Voprosy meditsinskoi khimii, 37(1), 16-19.