VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Production of a fibrinogen concentrate from small amounts of human autologous blood plasma and its short-term characteristics

   
Veremeenko K.N., Varetskaia T.V., Kizim A.I., Svital'skaia L.A.
PubMed Id: 1858340
Year: 1991  Volume: 37  Issue: 1  Pages: 44-46
Simple procedure was developed for production of fibrinogen concentrate from small amounts of human autogenous blood using precipitation with polyethylene glycol and ammonium sulfate. Maximal yield of fibrinogen was obtained using polyethylene glycol 2,000 and 6,000 daltons at concentrations 7% and 4.5%, respectively. The fibrinogen preparations included fibronectin, inhibitors of proteinases, factor XIII of blood coagulation, plasminogen. Sigma-Aminocapronic acid and contrical should be added during bloodletting as well as into blood plasma before isolation of fibrinogen to avoid possible fibrinogen proteolysis.
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Veremeenko, K. N., Varetskaia, T. V., Kizim, A. I., Svital'skaia, L. A. (1991). Production of a fibrinogen concentrate from small amounts of human autologous blood plasma and its short-term characteristics. Voprosy Meditsinskoi Khimii, 37(1), 44-46.
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