VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Accumulation of ATP by plasma membrane preparations enriched by particles from hypophyseal cells under the effect of thyrotropin-releasing hormone

   
Karelin A.A., Shumova E.A., Globa A.G., Zaĭtseva N.V., Titov M.I.
PubMed Id: 1650057
Year: 1991  Volume: 37  Issue: 1  Pages: 65-67
Thyrotropin-releasing hormone (TRH, thyroliberin) is endogenous tripeptide, which is synthesized in hypothalamus and is responsible for multihormonal regulation of synthesis and secretion of hormones in the anterior lobe of the hypophysis. TRH is required as an obligatory factor stimulating the GH cell lines growing in cell culture. As indicated in previous studies activation of target cells (in response to effects of insulin, growth hormone, polypeptide factors of growing, mitogens, chemostimulators) followed by fast alterations in phosphorylation of membrane components which is realized via generation of "signal" ATP in plasmatic membranes. This type of ATP was synthesized within 1 min in the preparations of particles enriched with plasmatic membranes isolated from bovine hypophyses and washed in 0.25 M sucrose, if the particles were incubated in the mixture containing TRH 30-40 mg/ml, Tris-HCl buffer, rH 7.5, ADP-Na salt Mg2+, inorganic phosphate, NaF, under conditions of NADH oxidation in presence of cytochrome c and oxygen. Accumulation of TRH-stimulated ATP in plasmatic membranes was detected, after addition of the kinases inhibitor 5'-fluorosulfonyl benzyladenosine into the incubation mixture, in II large scale experiments. The rate of TRH-stimulated synthesis of ATP was dissimilar in various preparations of hypophyses and was equal to 0.21-11.6 nmol/min/mg of protein at 30 degrees Plasmatic membrane signal ATP appears to serve as a secondary "membrane messenger" for TRH during transfer of a signal and its acceleration from the TRH receptors along the cell surface to membrane effectors--kinases.
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Karelin, A. A., Shumova, E. A., Globa, A. G., Zaĭtseva, N. V., Titov, M. I. (1991). Accumulation of ATP by plasma membrane preparations enriched by particles from hypophyseal cells under the effect of thyrotropin-releasing hormone. Voprosy Meditsinskoi Khimii, 37(1), 65-67.
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