VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Electrophoretic analysis of rat kidney glomerulus basal membrane

   
Khasanbaeva G.Sh., Khasigov P.Z., Rubachev P.G., Shishkin S.S.
PubMed Id: 1897204
Year: 1991  Volume: 37  Issue: 2  Pages: 86-90
A technique was developed for analysis of basal membrane proteins from rat kidney glomerulus using two-dimensional electrophoresis by O'Farrell. Basal membranes were isolated from the glomerulus by means of detergent treatment. Various procedures of basal membrane solubilization were studied. About 25 protein fractions with molecular mass from 25 kDa to 330 kDa were detected in the basal membrane after single-dimensional electrophoresis in presence of sodium dodecyl-sulfate. Two-dimensional electrophoresis of the basal membrane proteins and staining of gels with silver nitrate enabled to detect approximately 50 polypeptide fractions with molecular mass from 25 kDa up to 250 kDa at pH 5-7.
Download PDF:
Citation:

Khasanbaeva, G. Sh., Khasigov, P. Z., Rubachev, P. G., Shishkin, S. S. (1991). Electrophoretic analysis of rat kidney glomerulus basal membrane. Voprosy meditsinskoi khimii, 37(2), 86-90.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)