VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

A spectrophotometric method for determining the concentration of L-lysine using L-lysine-alpha-oxidase from Trichoderma sp. and 3,3',5,5'-tetramethylbenzidene dihydrochloride

   
Lukasheva E.V., Vesa V.S., Korpela T.K., Berezov T.T.
PubMed Id: 1949695
Year: 1991  Volume: 37  Issue: 3  Pages: 68-70
A simple and relatively sensitive procedure was developed for determination of L-lysine at 3-30 mmole/L concentration. The procedure does not involve the carcinogenic compound o-dianisidine. L-lysine alpha-oxidase catalyzed oxidative deamination of L-lysine with O2 consumption and formation of H2O2, NH3 and alpha-keto-epsilon-aminocaproic acid. Horseradish peroxidase and a non-carcinogenic compound 3,3,5,5'-tetramethylbenzidine dihydrochloride as an electron donor were used in determination of H2O2 formed. The procedure developed enabled also to measure the L-lysine alpha-oxidase activity at the enzyme concentrations of 10-500 ng/ml. The only limitation of the procedure is relatively low pH-values of the reaction medium.
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Lukasheva, E. V., Vesa, V. S., Korpela, T. K., Berezov, T. T. (1991). A spectrophotometric method for determining the concentration of L-lysine using L-lysine-alpha-oxidase from Trichoderma sp. and 3,3',5,5'-tetramethylbenzidene dihydrochloride. Voprosy Meditsinskoi Khimii, 37(3), 68-70.
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