VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Antigenic properties of lactate dehydrogenase isoenzymes of various biological types using antipeptide antibodies to a fragment of the active center of swine lactate dehydrogenase-M4

   
Grebenshchikova O.G., Prozorovskiĭ V.N.
PubMed Id: 1759391
Year: 1991  Volume: 37  Issue: 5  Pages: 21-24
Antigenic properties of human, pig, rabbit and rat lactate dehydrogenase (LDH) M4-isozyme as well as of human LDH-C4 were studied using antipeptide antibodies against pig LDH-M4 fragment (180-214). Amino acid sequence 180-214, containing His-195, which is involved in the active site of LDH isozymes, proved to be total antigenic determinant only for human and pig M4-isozymes. Amino acid sequence of total antigenic determinant did not allow any substitutions of essential amino acid residues. His-195, participating in substrate binding, was not involved immediately in reactions with antibodies as shown by means of chemical modification of pig M4-isozyme with diethyl pyrocarbonate and after production of antibodies against the modified isoenzyme.
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Grebenshchikova, O. G., Prozorovskiĭ, V. N. (1991). Antigenic properties of lactate dehydrogenase isoenzymes of various biological types using antipeptide antibodies to a fragment of the active center of swine lactate dehydrogenase-M4. Voprosy Meditsinskoi Khimii, 37(5), 21-24.
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