VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

An ultramicro method for determining pyrophosphatase using malachite green B

   
Gribanov G.A., Pankrushina A.N., Kirillova E.N.
PubMed Id: 8397457
Year: 1993  Volume: 39  Issue: 4  Pages: 58-59
A modified sensitive micromethod is described for estimation of pyrophosphatase activity both in homogenate and suspensions of nuclear and mitochondrial fractions of rat liver tissue using a kit of reagents containing malachite green B. The analysis may be carried out in the preparation containing only 0.2 mg of biological material; the procedure is reproducible, rapid and allows to the requisite amount of reagents to be decreased. The activity of pyrophosphatase in homogenate, nuclear and mitochondrial fractions of rat liver tissue was higher at acid pH values as compared with that in the neutral medium; the highest enzymatic activity was detected in the nuclear fraction.
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Gribanov, G. A., Pankrushina, A. N., Kirillova, E. N. (1993). An ultramicro method for determining pyrophosphatase using malachite green B. Voprosy Meditsinskoi Khimii, 39(4), 58-59.
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