VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Polarized fluoroimmunoanalysis of phenobarbital using the TD(x) analyzer from "Abbott" laboratories

   
Ermakov A.N., Bobrina D.E., Izotov B.N., Egorov A.M., Eremin S.A.
PubMed Id: 8379120
Year: 1993  Volume: 39  Issue: 4  Pages: 59-63
Polarographic fluoroimmunoassay was developed for identification and estimation of phenobarbital in urine. The study was carried out using TDx-analyzer "Abbott" (USA) under conditions of automatic service by modified program N 21 intended for analysis of kanamycin. In the procedure developed rabbit antiserum against phenobarbital was used at the initial dilution 1:2 and the tracer 5-ethyl-5-(4'-fluoroscein thiocarbamyl aminophenyl)barbituric acid was applied at the initial concentration of 60 nmol/l. The minimal content of phenobarbital evaluated in urine was as small as 0.09 microgram/ml and it was measured in the limits of 0.5 microgram/ml. Specificity of the analysis and its reproducibility were studied. The results of the polarographic fluoroimmunoassay correlated highly to the data of EMIT-st-assay and HPLC.
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Ermakov A.N., Bobrina D.E., Izotov B.N., Egorov A.M., Eremin S.A. (1993) Voprosy meditsinskoi khimii, 39(4), 59-63.
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