VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer

   
Mal'tsev G.Iu., Orlova L.A.
PubMed Id: 8160436
Year: 1994  Volume: 40  Issue: 2  Pages: 59-61
A relatively simple procedure was developed for estimation of the glutathione reductase activity using half-automatic analyzer. The procedure involved: preparation of hemolysates with minimal rate of erythrocyte destruction; substitution of buffers with high ionic strength for 0.05 M Tris-HCl buffer not containing EDTA. Most effective period of preincubation was found; dependence of the reaction rate on FAD was studied. The procedure developed was approved using two types of half-automatic analyzer and the model FP-901 was shown to be the most suitable for estimation of the glutathione reductase holoenzyme activity.
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Mal'tsev, G. Iu., Orlova, L. A. (1994). Optimization of determining human erythrocyte glutathione reductase activity using a semiautomatic analyzer. Voprosy meditsinskoi khimii, 40(2), 59-61.
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