Expression of cathepsins D and L during neoplastic transformation of fibroblasts

Dilakian E.A., Balaevskaia T.O., Zakamaldina-Tsama T.A., Topol L.Z., Solov'eva N.I.
PubMed Id: 8079433
Year: 1994  Volume: 40  Issue: 3  Pages: 2-6
Study of the expression of the aspartyl cathepsin D-like and cysteine cathepsin L-like proteinases was carried out on a model system of rat embryo fibroblasts. The model system employed makes it possible to distinguish two discrete successive stages of transformation in vitro: immortalization and tumorigenic transformation. The dynamics of expression and subcellular distribution of proteinases throughout the transformation process was followed. It was shown that in immortalized and transformed cells the activities of the aspartyl and cysteine proteinases were expressed to a variable degree and the expression was dependent on the time of cell cultivation. The increase in both the aspartyl cathepsin D-like proteinase and cysteine cathepsin L- and B-like proteinase activities was correlated with the stage of fibroblast transformation. At all stages studied of transformation, the major part of cathepsin L-like proteinase activity was localized within the cell, while among secreted proteinases the cathepsin D-like proteinase was apparently predominant. It was found that the secreted cathepsin D-like proteinase in all cell cultures studied was complexed with the inhibitor.
Download PDF:  

Dilakian, E. A., Balaevskaia, T. O., Zakamaldina-Tsama, T. A., Topol, L. Z., Solov'eva, N. I. (1994). Expression of cathepsins D and L during neoplastic transformation of fibroblasts. Voprosy Meditsinskoi Khimii, 40(3), 2-6.
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)