VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Detection of human leukocyte elastase from a plasma alpha-1-proteinase inhibitor complex by its enzymatic activity with a synthetic substrate

   
Dotsenko V.L., Neshkova E.A., Iarovaia G.A.
PubMed Id: 8079434
Year: 1994  Volume: 40  Issue: 3  Pages: 20-25
A spectrophotometric procedure was developed for estimation of elastase activity in human leukocytes in the form of complex with blood serum alpha 1 proteinase inhibitor after loosening of the complex and detection of the enzymatic activity with N-tert-butyl-hydroxy-carbonyl-Ala-p-nitrophenyl ester as a substrate in presence of acetone or acetonitrile. The optimal conditions were described, which were required for estimation of the enzyme 100% activity followed by addition of the leukocyte elastase standard preparation into blood serum as well as for measurement of the enzyme activity in the complex with alpha 1 proteinase inhibitor. The procedure took 6-10 min to evaluate the elastase activity in the complex with the inhibitor using simple buffer containing organic solvents at 30 degrees and the available two-beam spectrophotometer.
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Dotsenko, V. L., Neshkova, E. A., Iarovaia, G. A. (1994). Detection of human leukocyte elastase from a plasma alpha-1-proteinase inhibitor complex by its enzymatic activity with a synthetic substrate. Voprosy Meditsinskoi Khimii, 40(3), 20-25.
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