VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Determination of metabolism of steroid hormones and activity of their synthetic enzymes in murine Leydig cells by high performance liquid chromatography

   
Svechnikov K.V., Busygina T.V., Osadchuk A.V.
PubMed Id: 7975370
Year: 1994  Volume: 40  Issue: 4  Pages: 14-17
A very effective procedure was developed for separation of sex hormones of the delta 4 pathway using the microcolumn technique of high performance liquid chromatography (HPLC). A five-step gradient containing methanol, acetonitrile and water was used as a mobile phase, while Lychrosorb RP-18 with particles of 5 microns was used as a sorbent. Metabolism of steroid hormones and activity of 3 beta-steroid dehydrogenase, 17 alpha-hydroxylase, C17-20-lyase and 17-ketosteroid reductase were studied in Leydig cells of two mouse strains PT and A/He. The higher rate of steroid metabolism and of the enzymatic activity was detected in the mouse cells of PT strain as compared with those of A/He strain.
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Svechnikov K.V., Busygina T.V., Osadchuk A.V. (1994) Voprosy meditsinskoi khimii, 40(4), 14-17.
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