Erythrocytes of patients with insulin-dependent diabetes mellitus contained two forms of aldose reductase a and b (EC No. 188.8.131.52) (the key enzyme in the sorbitol pathway of glucose utilization) as compared with those of healthy donors in whom the form b of the enzyme was only found. The aldose reductase a exhibited a higher maximal rate (Vmax = 16.7 +/- 3.2 IU/D280) and a lower substrate affinity (Km = 6.5 = 19 mM) than the enzyme b, Vmax = 43.8 +/- 0.6 IU/D280, Km = 3.0 = 4.0 mM, respectively. More severe development of the disease was observed in the patients whose erythrocytes contained only aldose reductase a (HbA1c = 14.56 +/- 0.69%) as compared with those in whom the enzyme a and b were found (HbA1c = 11.5 +/- 0.4%). Kinetic parameters of the enzyme showed that aldose reductase a may be active in hyperglycemia of diabetes mellitus, thus contributing to intensification of the sorbitol pathway in these patients
Shono N.I. et al. Two forms of aldose reductase in erythrocytes from patients with insulin-dependent diabetes mellitus // Voprosy meditsinskoi khimii. - 1994. - V. 40. -N 5. - P. 45-48.
Shono N.I. et al., "Two forms of aldose reductase in erythrocytes from patients with insulin-dependent diabetes mellitus." Voprosy meditsinskoi khimii 40.5 (1994): 45-48.
Shono, N. I., Rabinovich, S. E., Platonova, L. V., Diuzheva, T. G. (1994). Two forms of aldose reductase in erythrocytes from patients with insulin-dependent diabetes mellitus. Voprosy meditsinskoi khimii, 40(5), 45-48.