VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Two forms of aldose reductase in erythrocytes from patients with insulin-dependent diabetes mellitus

   
Shono N.I., Rabinovich S.E., Platonova L.V., Diuzheva T.G.
PubMed Id: 7839671
Year: 1994  Volume: 40  Issue: 5  Pages: 45-48
Erythrocytes of patients with insulin-dependent diabetes mellitus contained two forms of aldose reductase a and b (EC No. 1.1.1.21) (the key enzyme in the sorbitol pathway of glucose utilization) as compared with those of healthy donors in whom the form b of the enzyme was only found. The aldose reductase a exhibited a higher maximal rate (Vmax = 16.7 +/- 3.2 IU/D280) and a lower substrate affinity (Km = 6.5 = 19 mM) than the enzyme b, Vmax = 43.8 +/- 0.6 IU/D280, Km = 3.0 = 4.0 mM, respectively. More severe development of the disease was observed in the patients whose erythrocytes contained only aldose reductase a (HbA1c = 14.56 +/- 0.69%) as compared with those in whom the enzyme a and b were found (HbA1c = 11.5 +/- 0.4%). Kinetic parameters of the enzyme showed that aldose reductase a may be active in hyperglycemia of diabetes mellitus, thus contributing to intensification of the sorbitol pathway in these patients
Download PDF:
Citation:

Shono, N. I., Rabinovich, S. E., Platonova, L. V., Diuzheva, T. G. (1994). Two forms of aldose reductase in erythrocytes from patients with insulin-dependent diabetes mellitus. Voprosy meditsinskoi khimii, 40(5), 45-48.
References
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)