The authors examined the human blood mononuclear-induced tumor necrosis factor production using the new drug--the synthetic hexapeptide Immunophan. The levels of tumor necrosis factor in the supernatant liquid were measured by the enzyme immunoassay and the cytotoxic test using L-929 fibroblastoid cells. Following 2-8 hours of short-term incubation of mononuclear cells with Immunophan, there was a reduction in spontaneous or lipopolysaccharide - or ionophore A23187-induced production of tumor necrosis factor. As high as 5-20% of plastic-nonadherent cells treated with Immunophan in a concentration of 0.25 mu/ml were found to produce the same effect. Two-four hours after Immunophan activation, the cells produced into the supernatant liquid soluble factors with a molecular weight of 70-85 kD that suppressed the production and activity of tumor necrosis factor. Thus, the modulating effect of Immunophan against tumor necrosis factor production is associated with the induction of regulatory cells producing the soluble receptors of tumor necrosis factor. It is suggested that extrabody pharmacological induction of the cells that regulate the production of tumor necrosis factor, followed by their subsequent administration into the autologic organism might be used while developing new variants of extracorporeal treatments of the diseases which are characterized by the pathogenetically significant hyperproduction of the inflammatory cytokins,--tumor necrosis factor, interleukin-1, interleukin-6.
Pisarev V.M. et al. Mechanisms of inhibiting production of tumor necrosis factor alpha by the synthetic hexapeptide--immunophane // Voprosy meditsinskoi khimii. - 1995. - V. 41. -N 2. - P. 11-15.
Pisarev V.M. et al., "Mechanisms of inhibiting production of tumor necrosis factor alpha by the synthetic hexapeptide--immunophane." Voprosy meditsinskoi khimii 41.2 (1995): 11-15.
Pisarev, V. M., Tutel'ian, A. V., Danilina, A. V., Kremlev, S. G., Tkacheva, T. D., Lebedeva, V. V., Pevnitsky, L. A. (1995). Mechanisms of inhibiting production of tumor necrosis factor alpha by the synthetic hexapeptide--immunophane. Voprosy meditsinskoi khimii, 41(2), 11-15.