The optimum conditions for extraction of catalytic active conjugates of alpha-amylase and antibodies for staphylococcal toxin (ST) have been created. The optimum correlation of antibodies and enzyme for the effective use of the extracted conjugate during enzyme immunoassay for ST has been established. The covalent immobilization of antibodies on the micropore polyamide membranes has been carried out. Apart from this, the extracted conjugated were purified by TSK-gel HN-55 chromatography. The optimum concentration of the conjugates has been defined.