In patients suffering from insulin-dependent diabetes mellitus (IDDM) with or without preclinical and clinical signs of diabetic nephropathy, the degree of epithelial cell lesions in the renal tubules was assessed from the urinary activities of enzymes at various sites, such as lysosomal (N-acetyl-beta-D-glucosaminidase (NAG) and beta-galactosidase (beta-GA)), brush edge membranous (alanine aminopeptidase (AAP), and cytosolic (alpha-glucosidase (alpha-GL)). Patients from Groups 1 and 2 had no preclinical and clinical signs of nephropathy. In Group 1 patients, the magnitude of enzymuria was not different from that in normalcy. However, Group 2 patients exhibited significant increases in urinary NAG and beta-GA activities as compared to Group 1 patients and healthy individuals. In Group 3 patients with microproteinuria from 0.05 to 0.5 mg protein per ml urine, displayed a further enhancement of NAG and beta-GA activities as compared to Group 2 patients and significantly higher activity than did Groups 1 and 2 patients and healthy individuals. In Group 4 patients with macroproteinuria of > 0.5 mg/ml), greater increases in the activities of NAG, beta-GA, and AAP were not found, however, there was a significant increase in alpha-G1 activity. The findings suggest the varying degrees of epithelial cell damage in the renal tubules in patients of different groups and the possibility of early detection of lesion in the proximal portion of nephronic tubules in IDDM patients as assessed from urinary enzyme levels.
Platonova L.V. et al. On urinary enzymes in insulin-dependent diabetis mellitus // Voprosy Meditsinskoi Khimii. - 1996. - V. 42. -N 1. - P. 70-76.
Platonova L.V. et al., "On urinary enzymes in insulin-dependent diabetis mellitus." Voprosy Meditsinskoi Khimii 42.1 (1996): 70-76.
Platonova, L. V., Rabinovich, S. E., Shono, N. I., Dyuzheva, T. G., Galperin, E. I. (1996). On urinary enzymes in insulin-dependent diabetis mellitus. Voprosy Meditsinskoi Khimii, 42(1), 70-76.
References
Бабаева Н.И., Липецкая И.Я., Творогова М.Г., Титов В.Н. // Лаб дело. -1991. — N 1. —С 9-16. Scholar google search
Базарова А.В., Мамаева Г.Г., Карелин А.А. // Пробл эндокринол. — 1990. — Т. 36, N 3.- С. 87-91. Scholar google search
Галенок В.А., Гостинская Е.В., Диккер B.E. Гемореология при нарушениях углеводного обмена. — Новосибирск, L987. Scholar google search
Дедов И.И., Мухин Н.А., Пальцев М.А. и др. // Тер. арх. - 1989. - Т. 61, N 12. -С. 73-76. Scholar google search
Делекторская Л.Н., Ертанов И.Д., Окунев Д.Ю. // Лаб. дело. — 1988. - N 9. — С. 3-8. Scholar google search
Иванов Ю.И., Погорелюк Л.Н. Обработка результатов медико-биологических исследований на микрокалькуляторах. — М., 1990. Scholar google search
Платонова Л.В., Шоно Н.И., Рабинович С.Э. // Клин, лаб диагн — 1995 — N 1. —С. 33-34. Scholar google search
Покровский А.А., Тутельян В.А. //Лизосомы — М., 1976. — С. 181. Scholar google search
Почки и гомеостаз в норме и при патологии / Под ред. С.Клара; Пер с англ — М., 1987. Scholar google search
Серов В.В. Пальцев М.А. // Почки и артериальная гипертензия. — М.. 1993. — С. 22. Scholar google search
Cantin М., Artizzu М., Luciano M., Gianetto R. // Рrос. Soc. exp. Biol. (NY ). - 1979 — Vol 162, N 1. - P. 121-127. Scholar google search
Hultberg В. // Clin. chim. Acta - 1980 - Vol. 108, N 2. - P. 195-199. Scholar google search
Jung K., Scholz D. // Clin. Chem. - 1980. - Vol 26, N 9. - P. 1251-1254. Scholar google search
Jung K., Pergande M., Schimke E. et al. // Ibid. — 1988 - Vol 34, N 3. — P. 544-547. Scholar google search
Miltenyi M., Korner A., Tulassay Т., Szabo A. //Arch. Dis. Childh. -1985 - Vol. 60, N 10.- P. 929-931. Scholar google search
Pesce M.A., Strande C.S. // Clin. Chem. - 1973. - Vol. 19, N 11. - P . 1265-1267. Scholar google search
Shimoji N., Kitahaski S., Naka K. et al. // Metabolism. - 1987. -Vol. 36, N 3. - P. 277-290. Scholar google search
Shoeberger G., Baser S., Nagraier V. et al. // Diagnostic Significance of Enzymes and Proteins in Urine // Eds. U.C. Dubach, U. Schmidt. - Bern, 1979. - P. 122-131. Scholar google search
Watanabe S., Asami Т., Sasazaki Y. et al. // Nephron. - 1982 - Vol. 32, N 3. - P. 296. Scholar google search
