The effect of human blood serum and several serum antioxidants (ascorbate, alpha-tocopherol, urate, albumin, transferrin and ceruloplasmin) on latent period and chemiluminescence (CL) intencity of hemoglobin-hydrogen peroxide-luminol system has been investigated. The addition of blood serum and antioxidants to the system increased the latent period and decreased CL intencity. The antioxidants had been taken in concentrations caused 50% inhibition of CL intencity of model system had not effected on the luminol photoluminescence. The possible mechanism of action of the antioxidants is discussed.
Teselkin Yu.O., Babenkova I.V., Lyubitsky O.B., Klebanov G.I., Vladimirov Yu.A. (1997) Inhibition by serum antioxidants of luminol oxidation in the presence of hemoglobin and hydrogen peroxide. Voprosy Meditsinskoi Khimii, 43(2), 87-93.
Teselkin Yu.O. et al. Inhibition by serum antioxidants of luminol oxidation in the presence of hemoglobin and hydrogen peroxide // Voprosy Meditsinskoi Khimii. - 1997. - V. 43. -N 2. - P. 87-93.
Teselkin Yu.O. et al., "Inhibition by serum antioxidants of luminol oxidation in the presence of hemoglobin and hydrogen peroxide." Voprosy Meditsinskoi Khimii 43.2 (1997): 87-93.
Teselkin, Yu. O., Babenkova, I. V., Lyubitsky, O. B., Klebanov, G. I., Vladimirov, Yu. A. (1997). Inhibition by serum antioxidants of luminol oxidation in the presence of hemoglobin and hydrogen peroxide. Voprosy Meditsinskoi Khimii, 43(2), 87-93.
References
Владимиров Ю.А., Азизова О.А., ДеевА.И. и др. Свободные радикалы в живых системах: Биофизика: Итоги науки и техники ВИНИТИ АН СССР. - М., 1991. - Т. 29. — С. 1- 252. Scholar google search
Vladimirov Y.A. // Free Radicals, Agigng and Degenerative Diseases. —New York, London: Alan R. Liss Inc., 1986. - P. 141-195. Scholar google search
Halliwell B. // Amer. J. Med. - 1991. - V. 91, Suppl. 3C. - P. 14-22. Scholar google search
O'Connell M.J.,Peters T.J. // Chem.and Phys.Lipids.- 1987— V.45, N 2-4.— P.241-249. Scholar google search
Bannister J.V., Bannister W. H., Hill H.A.O., Thormlley P.J. // Biochim. Biophys. Acta. — 1982. -V. 715, N l . - P . 116-120. Scholar google search
Motohashi N., Mori I. // FEBS Lett. - 1983. - V. 157, N 1. - P. 197-199. Scholar google search
Puppo A., Halliwell B. // Biochem. J. - 1988. - V. 249, N l . - P . 185-190. Scholar google search
Якушева Э.Ш., Осипов А. Н., Костенко О. В. и др. // Биофизика. — 1992. —Т. 37, N 6. — С. 1021-1028. Scholar google search
Nohl H, Stoke К. // Free Radic. Biol. Med. - 1993. - V. 15, N 3. - P . 257-263. Scholar google search
Yamana Т., Volkmer C, Grishan M.B. // Free Radic. Biol. Med. — 1991. - V. 10, N 1. — P. 41- 49. Scholar google search
Yamamoto Y., Ames B.N. // Free Radic. Biol. Med. - 1987. - V. 3, N 5. - P. 359-361. Scholar google search
Frei В., Stocker R., Ames B. // Proc. Natl. Acad. Sci. USA. - 1988. -V. 85, N 24. - P. 9748- 9752. Scholar google search
Bergmeyer H. U., Gawehn К, Grass/ M. // Methoden der Enzymatischen analyze / Ed. Bergmeyer H.U. - Velgar Chemie, Weinheim, 1970. - V. 1. - P . 440. Scholar google search
Faulkner K., Fridowich I. // Free Radic. Biol. Med. - 1993. - V. 15, N 4. - P. 447-451. Scholar google search
Iwaoka Т., Tobata F, Takahashi T. // Free Radic. Biol. Med. - 1987. - V. 3, N 5. - P. 329- 333. Scholar google search
Lissi E.A., Escobar J., Pascual C. et al. // Photchem. and Photobiol. — 1994. — V. 60, N 5. — P. 405-411. Scholar google search
Gutteridge J.M.C. //Chem.-Biol. Interactions. — 1985. — V. 56, N 1. — P. 113-120. Scholar google search
Шаронов Б.П., Говорова Н.Ю., Лызлова СИ. // Биохимия. — 1988. - Т. 53, N 5. — С. 816- 825. Scholar google search