VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

The method of estimation of lysozyme activity in serum with chitin azure as substrate

   
Pisarzhevskií S.A., Globa A.G., Shirshov O.N., Marchuk A.I., Svetuhin A.M., Karelin A.A.
PubMed Id: 9273783
Year: 1997  Volume: 43  Issue: 3  Pages: 185-190
The method of estimation of lysozyme activity in serum when chitin azure is used as substrate is described. The basis of incubation medium is 0,1 M acetate buffer, pH 5,0. It was judged about activity of lysozyme by fluorescence of azure in incubation medium at the end of three hour incubation at 37°C after separation of non-reacted chitin azure by short time centrifugation. It was shown that development of wound infection is accompanied by increasing of chitinase activity of lysozyme in serum.
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Keywords: lysozyme, serum, chitin azure, method of determination
Citation:

Pisarzhevskií, S. A., Globa, A. G., Shirshov, O. N., Marchuk, A. I., Svetuhin, A. M., Karelin, A. A. (1997). The method of estimation of lysozyme activity in serum with chitin azure as substrate. Voprosy meditsinskoi khimii, 43(3), 185-190.
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