VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Gene transfer into eucaryotic cells using non-cationic (neurtal) liposomes

   
Zhdanov R.I., Buneeva O.A., Podobed O.V., Kutsenko N.G., Tsvetkova T.A., Lavrenova T.P.
PubMed Id: 9312935
Year: 1997  Volume: 43  Issue: 4  Pages: 212-216
Transfection of plasmid DNAs containing b-galactosidase gene (pQE-LacZ) or alkaline phosphatase (pCSEAP) into L929 cell line using was studied. The complexes between plasmide DNA and liposomes containing Ca ions and glycyrrhizic acid or a- tocopherol caused successful transfection of functional genes into L929 cells. The efficiency of transfection of plasmid DNAs into L 929 cells using polynucleotide- metallo(II)-liposome complexes were 30-50% from the efficiency value of calcium phosphate coprecipitation transfection.
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Keywords: neutral liposomes, eucaryotic cells, lipofection, glycirhizic acid
Citation:

Zhdanov, R. I., Buneeva, O. A., Podobed, O. V., Kutsenko, N. G., Tsvetkova, T. A., Lavrenova, T. P. (1997). Gene transfer into eucaryotic cells using non-cationic (neurtal) liposomes. Voprosy meditsinskoi khimii, 43(4), 212-216.
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