VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Co-immobilization of L-lysine alpha-oxidase and peroxidase on porous membrane carriers

   
Lukasheva E.V., Rubtsova M.Ju., Kovba G.V., Berezov T.T., Egorov A.M.
PubMed Id: 9503574
Year: 1997  Volume: 43  Issue: 6  Pages: 566-575
The goal of the present study was the development of the optimal method of co-immobilization of two enzymes: L-lysine alpha-oxidase from Trichoderma sp. and horseradish peroxidase. Commercial nitrocellulose, nylon and N+ nylon membranes were used as carriers. The immobilization was carried out either by absorbtion or by covalent binding with aldehyde groups. The aldehyde groups were attached to the surface of the carriers by UV-irradiation of membranes in the presence of p-azidotetrafluorobenzaldehyde. The optimal concentrations of reagents, enzymes and reaction conditions were found. The membranes with the co-immobilised L-lysine alpha-oxidase and peroxidase were shown to be useful for the determination of L-lysine concentrations.
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Keywords: enzyme immobilization, peroxidase, L-lysine-alpha-oxidase, determination of L-lysine, membrane carriers
Citation:

Lukasheva, E. V., Rubtsova, M. Ju., Kovba, G. V., Berezov, T. T., Egorov, A. M. (1997). Co-immobilization of L-lysine alpha-oxidase and peroxidase on porous membrane carriers. Voprosy meditsinskoi khimii, 43(6), 566-575.
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