VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

The measuring of blood plasma antioxidant activity by the hemoglobin-hydrogen peroxide-luminol system

   
Teselkin Yu.O., Babenkova I.V., Lyubitsky O.B., Klebanov G.I., Vladimirov Yu.A.
PubMed Id: 9575615
Year: 1998  Volume: 44  Issue: 1  Pages: 70-76
The method of valuation of the blood plasma antioxidant activity (AOA) by the hemoglobin-hydrogen peroxide-luminol chemiluminescence system has been proposed. The method is based on the measuring the induction time of chemiluminescence that is directly proportional to the added volume of plasma or concentration of standard antioxidant. The ascorbate was taken as a standard. Blood plasma AOA has been expressed through the concentration of equivalent ascorbate solution (ascorbate equivalent). The influence of hemolysis and storage conditions of plasma on its detected AOA was investigated. The change of human blood plasma AOA after single administration of 2 g the ascorbate and its dynamics in patients with acute pancreatitis was studied.
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Keywords: free radical oxidation of luminol, chemiluminescence, blood plasma antioxidants, antioxidant activity
Citation:

Teselkin, Yu. O., Babenkova, I. V., Lyubitsky, O. B., Klebanov, G. I., Vladimirov, Yu. A. (1998). The measuring of blood plasma antioxidant activity by the hemoglobin-hydrogen peroxide-luminol system. Voprosy meditsinskoi khimii, 44(1), 70-76.
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