In the present work the effects of long-term prenatal gamma-irradiation (0.5 Gy) on the glucagon signalling through adenylyl cyclase have been investigated. In utero gamma-irradiation resulted in the increase of basal and GTP-stimulated adenylyl cyclase activity, whereas, the adenylyl cyclase response to glucagon was essentially reduced. The comparison of kinetic constants estimated from dose-response data for GTP and glucagon suggests that prenatal chronic irradiation prompted (i) the decrease in rate of GTP hydrolysis on Gs-protein; (ii) the reduction of glucagon potency to accelerate the exchange GDP for GTP on Gs-protein.
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Keywords: adenylyl cyclase, receptor, glucagon, Gs-protein, liver, in utero gamma-irradiation
Citation:
Chubanov V.S., Rogov I.I., Sholukh M.V., Konoplya E.F. (1998) Effects of prenatal gamma-irradiation on the functional properties of rat liver adenylyl cyclase. Voprosy Meditsinskoi Khimii, 44(2), 145-150.
Chubanov V.S. et al. Effects of prenatal gamma-irradiation on the functional properties of rat liver adenylyl cyclase // Voprosy Meditsinskoi Khimii. - 1998. - V. 44. -N 2. - P. 145-150.
Chubanov V.S. et al., "Effects of prenatal gamma-irradiation on the functional properties of rat liver adenylyl cyclase." Voprosy Meditsinskoi Khimii 44.2 (1998): 145-150.
Chubanov, V. S., Rogov, I. I., Sholukh, M. V., Konoplya, E. F. (1998). Effects of prenatal gamma-irradiation on the functional properties of rat liver adenylyl cyclase. Voprosy Meditsinskoi Khimii, 44(2), 145-150.
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