The method of enzyme linked immunosorbent assay (ELISA) for quantitative detection of chloramphenicol (CAP) in human blood serum was developed. Peculiarities of the adsorption on the microtitre plates surface of CAP-ovalbumin conjugate were investigated. Different conditions of competition stage of the analysis were studied. Conditions providing CAP monitoring in human blood serum in the clinical range were optimized. Matrix effect on the assay results was studied. The specificity of the analytical system was investigated and the reagents stability was examined. The method developed permits CAP concentration to be determined in human blood serum, diluted 1/100, in the linear range from 10 to 1000 ng/ml. The assay is characterized by high sensitivity (1 ng/mL) and good reproducibility (CV < 12%), assay time is about 3 hours.
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Keywords: drug monitoring, antibiotic therapy, chloramphenicol, enzyme immunoassay
Citation:
Kolosova A.Yu., Samsonova J.V., Blintsov A.N., Egorov A.M. (1998) Enzyme-linked immunosorbent assay of chloramphenikol in human blood serum. Voprosy Meditsinskoi Khimii, 44(2), 194-202.
Kolosova A.Yu. et al. Enzyme-linked immunosorbent assay of chloramphenikol in human blood serum // Voprosy Meditsinskoi Khimii. - 1998. - V. 44. -N 2. - P. 194-202.
Kolosova A.Yu. et al., "Enzyme-linked immunosorbent assay of chloramphenikol in human blood serum." Voprosy Meditsinskoi Khimii 44.2 (1998): 194-202.
Kolosova, A. Yu., Samsonova, J. V., Blintsov, A. N., Egorov, A. M. (1998). Enzyme-linked immunosorbent assay of chloramphenikol in human blood serum. Voprosy Meditsinskoi Khimii, 44(2), 194-202.
Chard T. An Introduction to Radioimmunoassay and Related Techniques. (1990). In: Laboratory Techniques in Biochemistry and Molecular Biology. (Burdon R.H., van Knippenberg P.H. Eds.) Elsevier: Amsterdam - New York - Oxford. 6. Part II. Scholar google search
