Using immunoblotting with specific antibodies, we have identified b1- and b 2-subunits of Gi-proteins in membrane andcytosolic fractions of pig lung. It has been shown that b1-subunit is present both in membrane and cytosolic fractions, whereas b2-subunit is associated only with membranes. Activation of membrane-bound G proteins withnon-hydrolisable GTP analogues have led to partial release from membrane of b 1-, but not b 2-subunits. Whendepolimerisation of F-actin during fractionation was prevented, both b1- and b 2-subunits were found in fraction containing totalmembranes and F-actin. Dialysis of this fraction into low ionic strength buffer causeddepolimerization of the bulk of actin and release of about 1/4 of b1-subunits into solution. The data presented here suggest that distribution of b 1-subunits between membrane and cytosol could depend on the stateof actin cytoskeleton.
Grishin A.V., Gerasimovskaya E.V., Starikova M.G., Panchenko M. P., Nickashin A. V., Tkachuk V. A. (1998) Interaction of b 1 subunit of G proteins with actin cytoskeleton. Voprosy Meditsinskoi Khimii, 44(4), 347-352.
Grishin A.V. et al. Interaction of b 1 subunit of G proteins with actin cytoskeleton // Voprosy Meditsinskoi Khimii. - 1998. - V. 44. -N 4. - P. 347-352.
Grishin A.V. et al., "Interaction of b 1 subunit of G proteins with actin cytoskeleton." Voprosy Meditsinskoi Khimii 44.4 (1998): 347-352.
Grishin, A. V., Gerasimovskaya, E. V., Starikova, M. G., Panchenko, M. , P., Nickashin, A. , V., Tkachuk, V. , A. (1998). Interaction of b 1 subunit of G proteins with actin cytoskeleton. Voprosy Meditsinskoi Khimii, 44(4), 347-352.
