Using immunoblotting with specific antibodies, we have identified b1- and b 2-subunits of Gi-proteins in membrane andcytosolic fractions of pig lung. It has been shown that b1-subunit is present both in membrane and cytosolic fractions, whereas b2-subunit is associated only with membranes. Activation of membrane-bound G proteins withnon-hydrolisable GTP analogues have led to partial release from membrane of b 1-, but not b 2-subunits. Whendepolimerisation of F-actin during fractionation was prevented, both b1- and b 2-subunits were found in fraction containing totalmembranes and F-actin. Dialysis of this fraction into low ionic strength buffer causeddepolimerization of the bulk of actin and release of about 1/4 of b1-subunits into solution. The data presented here suggest that distribution of b 1-subunits between membrane and cytosol could depend on the stateof actin cytoskeleton.
Grishin A.V., Gerasimovskaya E.V., Starikova M.G., Panchenko M. P., Nickashin A. V., Tkachuk V. A. (1998) Voprosy meditsinskoi khimii, 44(4), 347-352.
Grishin A.V. et al. Interaction of b 1 subunit of G proteins with actin cytoskeleton // Voprosy meditsinskoi khimii. - 1998. - V. 44. -N 4. - P. 347-352.
Grishin A.V. et al., "Interaction of b 1 subunit of G proteins with actin cytoskeleton." Voprosy meditsinskoi khimii 44.4 (1998): 347-352.
Grishin, A. V., Gerasimovskaya, E. V., Starikova, M. G., Panchenko, M. , P., Nickashin, A. , V., Tkachuk, V. , A. (1998). Interaction of b 1 subunit of G proteins with actin cytoskeleton. Voprosy meditsinskoi khimii, 44(4), 347-352.