Technologies based on the use of polymerase chain reaction, in particular IS PCR are widely used now. However, wide introduction in clinico-laboratory practice of these methods requires: increase of reliability of the accepted decisions, improvement of the image for revealing more fine distinctions of objects, increase of quality of the obtained information, automated registration of results of the analysis. The application of original digital image analysis, adapted to specificity of biomedical objects--morphodensitometry--allows to solve successfully the following tasks: to optimisze conditions of IS PCR realisation on the basis of use of quantitative opticogeometrical parameters, quantitatively and subpopulistically to characterize a degree of infected cells with viral infection, and also nucleuses of cells, that is especially actual for introduction of molecular-genetic methods in clinico diagnostic practice and, automatically to register results of the analysis. Thus, employment of morphodensitometry can be very important in genediagnostic and also gene therapy.
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Keywords: computer morphodensitometry, digital image analysis, IS PCR, hepatitis, distribution amplicon
Citation:
Kogan E.M., Zhukotsky A.V., Govorun V.M., Momynaliev K.T., Potapov I.A., Yakubova N.I., Yalpaev V.V. (1998) The use of computer morphodensitometry in modern molecular-diagnostic practice. Voprosy Meditsinskoi Khimii, 44(6), 527-536.
Kogan E.M. et al. The use of computer morphodensitometry in modern molecular-diagnostic practice // Voprosy Meditsinskoi Khimii. - 1998. - V. 44. -N 6. - P. 527-536.
Kogan E.M. et al., "The use of computer morphodensitometry in modern molecular-diagnostic practice." Voprosy Meditsinskoi Khimii 44.6 (1998): 527-536.
Kogan, E. M., Zhukotsky, A. V., Govorun, V. M., Momynaliev, K. T., Potapov, I. A., Yakubova, N. I., Yalpaev, V. V. (1998). The use of computer morphodensitometry in modern molecular-diagnostic practice. Voprosy Meditsinskoi Khimii, 44(6), 527-536.
References
Komminoth P., Long P.P. (1993). Virchows Archiv B Cell Pathology. 64:67-73;. Scholar google search
Nuovo G.J., Gallery K, MacConnell P., Braun A. (1994). Am. J. Pathol. 144. 659-666. Scholar google search
Жукоцкий А.В. (1992). Компьютерная телевизионная морфоденситометрия нормальных и патологических клеток и тканей в норме и патологии: Автореф. дисс. докт. мед. наук. -Москва. Scholar google search
Жукоцкий А.В., Якубова Н.И., Пономарев А.В., Пуртов В.А., Пальцев М.А.,Коган Э.М. (1992). Бюл. эксперим. биологии и медицины. Т. СШХ. -N.4, 417-419. Scholar google search
Zhukotsky A. V., Molodtsov M. U., Butusova N.N. et. al. (1990). Laser applications in life sciences. The 3-rd Int. Conf., Book of abstr. Moscow, v. 2, p. 74. Scholar google search
A.V. Zhukotsky, M. UMolodstov, N.N.Butusova, N.I. Yakubova, EM.Kogan. (1990). Laser application in life sciencies. The 3 Int. Conf. on Laser Scattering Spectroscopy and Diagnostics of Biological Objects, Book of abstr. vol 2, pp. 74. Scholar google search
Жукоцкий А.В., Якубова Н.И., Никулина Л.А., Королев Ю.Н., Зубкова СМ. (1993). Биофизика, 38. -Вып. 2. 284-286. Scholar google search
Zhukotsky А. V., Erenpreisa Je.A. (1992) Proc. the Latvian Acad. Sci. B. N. 7(540), 74-75. Scholar google search
A. Zhukotsky, V. Kopylov, O. Marchenko, O. Lomakin, S. Chemysh, T. Putina, E. Kogan. (1994). J.'Analyt. Cell. Pathol., 3, 218. Scholar google search
Zhukotsky A., Yevstigneeva M., Mishnev O., Kogan E. (1994). J. Analyt. Cell. Pathol., 3, 218. Scholar google search
Batzer MA., Deininger P.L., Hellmann-Blumberg U., Jurka J., Labuda D., Rubin СМ., Schmid CW. (1996). J. Mol. Evol., 42: 3-6. Scholar google search
Жукоцкий А .В., Бутусова Н.Н., Щеголев А .И., Коган Э.М. (1985). Автоматизация цитологических исследований: Сб. науч. тр. / АН СССР. Науч. центр биол. исслед. ИБФ. - Пущино,. 78-87. Scholar google search
Жукоцкий А.В., Елютина СИ., Молодцов М.Ю., Бутусова Н.Н., Коган Э.М. (1990). II Всесоюз. научно-практ. конф. молодых спец. Применение вычислительной техники в медицине, г. Москва 28-29 нояб. 1990 г.: Тез. докл. М., 156-157. Scholar google search
Zhukotsky A., Kogan К, Kopylov V., Marchenko О., Lomakin О. (1994) Biochemical Diagn. Instrument. (Editors R.F.Bonner, G.E.Cohn, Th.M.Laue, A.V.Priezzhev.) Proc. SPIE, Los-Angeles, 2136, pp. 36-42,. Scholar google search
Zhukotsky A. V., Kogan EM. (1989). In Sov. Med. Rev. B. Physicochem Asp. Med. - Harwood Academic Publishers GmbH, 2, pp.25-77. Scholar google search
