The two-site enzyme-linked immunosorbent assay (ELISA) for lactoferrin using polyclonal antibodies to spatially distant epitopes has been developed. The assay sensitivity defined as minimal detectable lactoferrin concentration for p = 0.05 is 0.5 ng/ml. Accuracy of the assay (variance coefficient) is 7% within the clinical range of antigen concentrations. Human albumin, hemoglobin, and transferrin in concentrations up to 5 mg/ml practically do not interfere with the measurement. Sera of healthy donors and viral hepatitis patients were investigated using the two-site ELISA. The lactoferrin content in 44 donors' sera was 130 +/- 40 ng/ml (medium +/- standard deviation). A study of the serum specimens of 95 patients with hepatitis A, B, and C showed significant increase in serum lactoferrin concentration: 850 +/- 420, 780 +/- 580, and 680 +/- 500 ng/ml respectively. The assay showed good characteristics and may be recommended for lactoferrin measurement in patients' sera.
Laktionov P. P., Shevchuk N.A., Naumov V. A., Zhevachevsky N.G., Rykova E.Yu., Vlasov V. V. (1999) Voprosy meditsinskoi khimii, 45(2), 170-177.
Laktionov P. P. et al. Development of a two-site immunoassay using polyclonal antibodies for lactoferrin measurement in human sera // Voprosy meditsinskoi khimii. - 1999. - V. 45. -N 2. - P. 170-177.
Laktionov P. P. et al., "Development of a two-site immunoassay using polyclonal antibodies for lactoferrin measurement in human sera." Voprosy meditsinskoi khimii 45.2 (1999): 170-177.
Laktionov, P. , P., Shevchuk, N. A., Naumov, V. , A., Zhevachevsky, N. G., Rykova, E. Yu., Vlasov, V. , V. (1999). Development of a two-site immunoassay using polyclonal antibodies for lactoferrin measurement in human sera. Voprosy meditsinskoi khimii, 45(2), 170-177.