VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Development of a two-site immunoassay using polyclonal antibodies for lactoferrin measurement in human sera

   
Laktionov P. P., Shevchuk N.A., Naumov V. A., Zhevachevsky N.G., Rykova E.Yu., Vlasov V. V.
PubMed Id: 10378308
Year: 1999  Volume: 45  Issue: 2  Pages: 170-177
The two-site enzyme-linked immunosorbent assay (ELISA) for lactoferrin using polyclonal antibodies to spatially distant epitopes has been developed. The assay sensitivity defined as minimal detectable lactoferrin concentration for p = 0.05 is 0.5 ng/ml. Accuracy of the assay (variance coefficient) is 7% within the clinical range of antigen concentrations. Human albumin, hemoglobin, and transferrin in concentrations up to 5 mg/ml practically do not interfere with the measurement. Sera of healthy donors and viral hepatitis patients were investigated using the two-site ELISA. The lactoferrin content in 44 donors' sera was 130 +/- 40 ng/ml (medium +/- standard deviation). A study of the serum specimens of 95 patients with hepatitis A, B, and C showed significant increase in serum lactoferrin concentration: 850 +/- 420, 780 +/- 580, and 680 +/- 500 ng/ml respectively. The assay showed good characteristics and may be recommended for lactoferrin measurement in patients' sera.
Download PDF:
Keywords: lactoferrin, two-site immunoassay, blood serum, viral hepatitis
Citation:

Laktionov, P. , P., Shevchuk, N. A., Naumov, V. , A., Zhevachevsky, N. G., Rykova, E. Yu., Vlasov, V. , V. (1999). Development of a two-site immunoassay using polyclonal antibodies for lactoferrin measurement in human sera. Voprosy meditsinskoi khimii, 45(2), 170-177.
References  
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)