VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Mechanisms regulation activity of nitric oxide synthase by calmodulin: a reveiw

   
Gervaziev Yu.V., Sokolov N.N.
Year: 1999  Volume: 45  Issue: 3  Pages: 187-199
The Ca2+-calmodulin system controls the neuronal and endotelial isoforms of NOS, whereas the inducible isoform is calcium independent apparently because CaM is a tightly bound subunit of iNOS. The canonical CaM-binding site is located between the oxygenase and reductase NOS domains. CaM controls eNOS dimerization rather then iNOS one. The proteins with the so-called “IQ” motif bind calmodulin in a Ca2+-independent manner. This group of proteins does not include iNOS, which has the canonical CaM-binding motif. In the experiments with synthetic peptides was demonstrated that the interaction between the calmodulin and CaM-binding site of iNOS does not depend on the Ca2+ concentration. On the other hand, in the experiments with fusion, mutant and truncated NOSs was shown that these features of CaM-binding region of iNOS is not enough for the enzyme to bind calmodulin Ca2+-independently; this interaction requiers the additional binding sites both in reductase and oxygenase domains of iNOS. In the experiments with fusion calmodulins the mechanism of calmodulin regulation of electron transfer in NOS was elaborated. The concept of autoinhibitory control element in the FMN-binding site of constitutive NOS is discussed
Download PDF:
Reference:

Gervaziev Yu.V., Sokolov N.N. (1999) Voprosy meditsinskoi khimii, 45(3), 187-199.
References  
 2002 (vol 48)
 2001 (vol 47)
 2000 (vol 46)
 1999 (vol 45)
 1998 (vol 44)
 1997 (vol 43)
 1996 (vol 42)
 1995 (vol 41)
 1994 (vol 40)
 1993 (vol 39)
 1992 (vol 38)
 1991 (vol 37)
 1990 (vol 36)
 1989 (vol 35)
 1988 (vol 34)
 1987 (vol 33)
 1986 (vol 32)
 1985 (vol 31)
        

© Institute of Biomedical Chemistry, Moscow, Russia