In vivo stability and distribution of deoxyribooligonucleotides and 3'-end modified oligodeoxyribooligonucleotides were studied in blood serum and cells. Substitution of two 3'-end internucleotide phosphodiester bonds increased the stability of modified oligonucleotides. Modified oligonucleotides were shown to be stable in blood serum for up to 24 hours. Leukocytes did not bind oligonucleotides significantly, whereas erythrocytes accumulate mono-, di-, three-, tetranucleotides appeared in the blood stream as degradation products of the parent oligonucleotide.
Laktionov P.P., Bryksin A.V., Rykova E.Yu., Amirchanov N. V., Vlasov V. V. (1999) Pharmacokinetic sand stability of phosphodiester and phosphorothioate 3`end modified oligonucleotides inthe blood in vivo: a comparative study. Voprosy Meditsinskoi Khimii, 45(3), 206-214.
Laktionov P.P. et al. Pharmacokinetic sand stability of phosphodiester and phosphorothioate 3`end modified oligonucleotides inthe blood in vivo: a comparative study // Voprosy Meditsinskoi Khimii. - 1999. - V. 45. -N 3. - P. 206-214.
Laktionov P.P. et al., "Pharmacokinetic sand stability of phosphodiester and phosphorothioate 3`end modified oligonucleotides inthe blood in vivo: a comparative study." Voprosy Meditsinskoi Khimii 45.3 (1999): 206-214.
Laktionov, P. P., Bryksin, A. V., Rykova, E. Yu., Amirchanov, N. , V., Vlasov, V. , V. (1999). Pharmacokinetic sand stability of phosphodiester and phosphorothioate 3`end modified oligonucleotides inthe blood in vivo: a comparative study. Voprosy Meditsinskoi Khimii, 45(3), 206-214.
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