VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Loss of serotonin transporter immunoreactive 43-kDa protein in platelets from patients with somatoform disorders correlates with depressive symptoms

   
Belous A.R., Ramamoothy S., Blakely R.D., Factor M.I., Lozier R.Y., Dupin A.M., Bechiashvili A.G., Brusov O.S., Morozova M.A.
PubMed Id: 10432563
Year: 1999  Volume: 45  Issue: 3  Pages: 256-262
To elucidate the role of serotonin transporter (SERT) protein as a candidate target for somatoform disorders (SD), we have performed an association study of the SERT immunoreactive (IR-SERT) protein level by using site-specific antibodies directed at C-terminus epitope poorly conserved among other cotransporters. 22 patients who met DSM-IY criteria for SD and 32 healthy volunteers matched in sex and age participated in the study. In platelets from all healthy donors, IR-SERT proteins migrates as a diffuse band between 75 and 102 kDa and a major sharper band at 43 kDa. We revealed almost complete disappearance of platelet 43 kDa IR-SERT protein in all patients with SD. All patients were rated with Hamilton Depressive Rating Scale (HDRS). There were found a significant negative correlation between residual IR-SERT 43 kDa protein level and HDRS Scores [r = -0.83; P < 0.000001]. These findings suggest that a biosynthetic or processing abnormality may exist in SERTs in the affected population and may reflect the dysfunction of serotonin neurotransmission in CNS.
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Belous A.R., Ramamoothy S., Blakely R.D., Factor M.I., Lozier R.Y., Dupin A.M., Bechiashvili A.G., Brusov O.S., Morozova M.A. (1999) Voprosy meditsinskoi khimii, 45(3), 256-262.
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