VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Conjugation of L-lysine ?-oxidase with antibodies

   
Gavrilova E.M.1, Smirnova I.P.2, Egorov A.M.1, Berezov T.T.2, Gogichaeva N.V.2, Lukasheva E.V.2

1. Moscow State Lomonosov University
2. People's Friendship University, Moscow
PubMed Id: 11075424
Year: 2000  Volume: 46  Issue: 4  Pages: 410-418
The conjugation of the drugs with vector molecules enables to obtain therapeutic preparation, which may be transported to the selected target organ. In the present work the methods of conjugation of antineoplastic enzyme L-lysine alpha-oxidase with antibodies were elaborated. Conjugates were worked out through the attachment of amino groups on the antibody surface either with the aldehyde groups which were created in L-lysine alpha-oxidase molecule (0.2% of initial enzymatic activity) or with the aldehyde groups of cross-linking molecules. Maximal (78%) L-lysine alpha-oxidase activity in conjugates was observed when oxidized peroxidase which contained the aldehyde groups was used as crosslinking agent. The glutaraldehyde method yielded 70% of initial enzyme activity.
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Keywords: L-lysine alpha-oxidase, peroxidase, antibodies, conjugate, directed transport
Citation:

Gavrilova, E. M., Smirnova, I. P., Egorov, A. M., Berezov, T. T., Gogichaeva, N. V., Lukasheva, E. V. (2000). Conjugation of L-lysine ?-oxidase with antibodies. Voprosy meditsinskoi khimii, 46(4), 410-418.
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