VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Methodological aspects of the employment of n-(3-[2-furyl]acryloyl)-phe-gly-gly for photometric assay of angiotensinconverting enzyme activity

   
Altchouler B.Y.1

1. Bauman Clinical Hospital №29
PubMed Id: 11234287
Year: 2000  Volume: 46  Issue: 6  Pages: 626-640
Different aspects of photometric assay of angiotensin converting enzyme (ACE) with synthetic cromogenic substrate N-(3-[2-furyl]-acryloyl)-phe-gly-gly (FAPGG) were evaluated. At FAPGG concentration exceeding 0.025 mM the absorbance does not follow Burger-Lambert-Bar dependences. Under three conditions the rate of absorbance of the reaction medium strongly depends on properties of spectral weight band. This explains dramatic interamlyser variations and complicetes standardization of the method. Linear dependence between photometic response and ACE activity. The employment of special inhibitor be recommended for elimination of interference.
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Reference:

Altchouler B.Y. (2000) Voprosy meditsinskoi khimii, 46(6), 626-640.
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